Localization of the simian virus 40 small t antigen in the nucleus and cytoplasm of monkey and mouse cells.
نویسندگان
چکیده
Monkey and mouse cells producing simian virus 40 small t antigen in the absence of clearly detectable intact or truncated large T antigens were subjected to indirect immunofluorescence and biochemical cell compartment analyses. Results revealed specific immunofluorescence and small t polypeptide in both the nucleus and cytoplasm of these cells.
منابع مشابه
Ultrastructural Study of Rotavirus Replication and Localization of the Intermediate Capsid Protein VP6
Rotavirus, a triple-layered non-enveloped member of the Reoviridae family, obtained a transient membrane envelope when newly synthesized subviral particles bud into the endoplasmic reticulum (ER). As rotavirus particles mature, they lose their transient membrane and obtain outer layer. It is mostly believed that only double layered particles bud into the ER. The present study describes that the...
متن کاملImmunocytochemical localization of T antigen in cells of BK virus-induced hamster brain tumor.
The localization of both the large T and small t tumor (T) antigens in cultured cells (Vn 12 cells) of hamster brain tumors induced with BK virus (BKV), a new human papovavirus, was studied by an enzyme labelled antibody method at both the light and electron microscopic levels. Under the light microscope, BKV T antigen was observed in the nucleus, except for the nucleoli, of cells in interphase...
متن کاملVariants of defective simian papovavirus 40 (PARA) characterized by cytoplasmic localization of simian papovavirus 40 tumor antigen.
Three isolates of PARA (particle aiding replication of adenovirus)-adenovirus 7 out of a total of 112 clonal progeny derived by two successive plaque purifications in green monkey kidney cells (GMK) were found to induce the synthesis of simian papovavirus40 (SV 40) tumor (T) antigen in the cytoplasm of infected cells. The variant viruses induced plaque formation in human embryonic kidney cells ...
متن کاملImmunocytochemical localization of simian virus 40 T antigen with peroxidase-labeled antibody fragments.
T antigen was localized in simian virus 40 lytically infected and transformed cells by Fab' antibody conjugates at the ultrastructural level. This virus-specific protein appeared in the cytoplasm of permissive cells as early as 3 h after infection. At later time intervals, the T antigen was localized in both the cytoplasm and nucleus and finally (24 h) in the nucleus. These results suggest a sy...
متن کاملComparison of Nucleus and Cytoplasm Diameter of Buccal Mucosa Cells in Cigarette Smokers and Nonsmokers: A Cytomorphometric Study using Feulgen and Papanicolaou Stains
Introduction: A decrease in cell size and an increase in nuclear dimension due to changes in the amount of DNA could be an indicator of early detection of malignant changes. This study aimed to compare the nuclear and cytoplasmic diameters of buccal mucosa cells in cigarette smokers and nonsmokers in a cytomorphometric study using Feulgen and Papanicolaou stains. Material & Methods: In this cas...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of virology
دوره 50 2 شماره
صفحات -
تاریخ انتشار 1984